Richards A, Coote G E, Pearce E I
Department of Oral Anatomy, Dental Pathology and Operative Dentistry, Royal Dental College, Faculty of Health Sciences, Aarhus University, Denmark.
J Dent Res. 1994 Mar;73(3):644-51. doi: 10.1177/00220345940730031001.
An acid-etch technique and a proton probe were used to obtain profiles of changes in fluoride concentrations across enamel of 24 normal and 24 fluorotic porcine teeth. Polished and unpolished sections were used for probe measurements, and blocks of enamel cut from tissue adjacent to the sections were used for acid etching. Additional blocks were etched with acid containing dye to study penetration of acid beyond the sampling sites. Probe-derived values were characterized by wide fluctuations. They were also higher than acid-etch values, and this difference was reduced but not eliminated by polishing the sections prior to scanning. Profiles obtained with the two methods followed a parallel course in fluorotic and normal enamel. Thus, errors due to chemical estimations of enamel depth with the etch technique were not demonstrated, although penetration of acid deep to the surface of the porous fluorotic enamel was observed. Further development of the precision of the probe method is required to optimize the unique advantage provided by the potential accuracy, speed of data collection, and spatial resolution of this method.
采用酸蚀技术和质子探针获取了24颗正常猪牙和24颗氟斑牙牙釉质中氟浓度的变化曲线。使用抛光和未抛光的切片进行探针测量，从与切片相邻的组织中切下的牙釉质块用于酸蚀。用含染料的酸蚀刻额外的牙釉质块，以研究酸在采样部位之外的渗透情况。探针得出的值波动较大。它们也高于酸蚀值，并且在扫描前对切片进行抛光可使这种差异减小，但不会消除。在氟斑牙和正常牙釉质中，用两种方法获得的曲线走势平行。因此，尽管观察到酸深入到多孔氟斑牙釉质表面，但未证明用蚀刻技术对牙釉质深度进行化学估计会产生误差。需要进一步提高探针方法的精度，以优化该方法潜在的准确性、数据收集速度和空间分辨率所提供的独特优势。
