Uchida T
Department of Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1994 Mar;61(1):44-55. doi: 10.5357/koubyou.61.44.
Local metabolic balance between the bone formation and resorption is modulated by many growth factors present in the bone. The mechanism of this regulation has been investigated by many researchers but is not completely clarified yet. In this study, the author found the presence of several growth factors in the 1 M NaCl extract from the bovine bone and one of them was confirmed to be different from the factors previously reported in the bone tissue. This factor was purified by means of gel filtration and heparin-affinity chromatography and characterized in detail. The purified factor stimulated the proliferation of the fibroblast cell line, Balb/c3T3, but neither stimulated nor inhibited that of the osteoblast cell line, MC3T3-E1. The factor was stable against heat (65 degrees C), acid (2N HCl), 6M urea or 4M guanidine-HCl treatments. However, it was inactivated by trypsin. The molecular size of this factor was about 15,000 and had a high affinity for heparin. In contrast to aFGF or bFGF, the fibroblast-proliferating activity of this factor was suppressed dose-dependently by heparin addition. According to those properties, this factor was indicated to be different from TGF- beta, aFGF, bFGF, PDGF, IGF-I, IGF-II or BMP which were reported to be present in the bone tissue. It is suggested the presence of a new factor in the bone which may regulate the proliferation of fibroblasts in vivo.
骨形成与吸收之间的局部代谢平衡受骨中存在的多种生长因子调节。许多研究人员对这种调节机制进行了研究，但尚未完全阐明。在本研究中，作者发现牛骨1M NaCl提取物中存在几种生长因子，其中一种被证实与先前报道的骨组织中的因子不同。该因子通过凝胶过滤和肝素亲和层析进行纯化，并进行了详细表征。纯化后的因子刺激成纤维细胞系Balb/c3T3的增殖，但对成骨细胞系MC3T3-E1既不刺激也不抑制。该因子对热（65℃）、酸（2N HCl）、6M尿素或4M盐酸胍处理稳定。然而，它被胰蛋白酶灭活。该因子的分子大小约为15000，对肝素有高亲和力。与aFGF或bFGF不同，添加肝素后该因子的成纤维细胞增殖活性呈剂量依赖性抑制。根据这些特性，该因子被认为不同于据报道存在于骨组织中的TGF-β、aFGF、bFGF、PDGF、IGF-I、IGF-II或BMP。提示骨中存在一种新因子，可能在体内调节成纤维细胞的增殖。
