Abe M, Ozaki S, Wolfenbarger D, deBram-Hart M, Weiss D T, Solomon A
Department of Medicine, University of Tennessee Medical Center/Graduate School of Medicine, Knoxville.
J Clin Lab Anal. 1994;8(1):4-9. doi: 10.1002/jcla.1860080103.
The distribution of the major VL subgroups (V lambda I, V lambda II, V lambda III, V lambda IV, V lambda VI, and V lambda VIII) among lambda-type immunoglobulins (Igs) in normal serum was determined by a sandwich enzyme-linked immunosorbent assay (ELISA) using a panel of murine anti-human V lambda-subgroup-specific monoclonal antibodies (MoAbs) and appropriate reference standard proteins. The mean concentration of lambda I, lambda II, lambda III, lambda IV, lambda VI, and lambda VIII Igs in serum specimens obtained from 23 adults was 2158, 162, 1958, 264, 225, and 169 micrograms/mL and represented 44, 3, 40, 5, 5, and 3% of the total Ig lambda population, respectively. The low percentage of lambda II Igs in normal serum was in marked contrast to the approximately 40% incidence of this V lambda subgroup found among lambda-type Bence Jones proteins and monoclonal serum Igs obtained from patients with multiple myeloma and AL amyloidosis, or approximately 60% in Waldenström's macroglobulinemia. The non-random expression of the V lambda II subgroup in these diseases implies a relationship between V lambda-gene usage and plasma cell, as well as certain types of lymphocytic dyscrasias.
使用一组鼠抗人Vλ亚组特异性单克隆抗体（MoAbs）和适当的参考标准蛋白，通过夹心酶联免疫吸附测定（ELISA）确定正常血清中λ型免疫球蛋白（Igs）中主要Vλ亚组（VλI、VλII、VλIII、VλIV、VλVI和VλVIII）的分布。从23名成年人获得的血清标本中，λI、λII、λIII、λIV、λVI和λVIII Igs的平均浓度分别为2158、162、1958、264、225和169微克/毫升，分别占总Igλ群体的44%、3%、40%、5%、5%和3%。正常血清中λII Igs的低百分比与在多发性骨髓瘤和AL淀粉样变性患者的λ型本-周蛋白和单克隆血清Igs中发现的该Vλ亚组约40%的发生率形成显著对比，在华氏巨球蛋白血症中约为60%。VλII亚组在这些疾病中的非随机表达意味着Vλ基因使用与浆细胞以及某些类型的淋巴细胞发育异常之间存在关联。
