Yamasaki T, Akiyama Y, Kawahara M, Nagao S, Moritake K, Enomoto K, Maeno T
Department of Neurosurgery, Shimane Medical University, Izumo, Japan.
No Shinkei Geka. 1994 Apr;22(4):327-31.
Examination was made of the effects of nilvadipine on membrane potential in cultured neuroblastoma-glioma hybrid NG108-15 cells using the current clamp method. The NG108-15 cells line differentiates into neuronal cells following the addition of 1mM dibutyryl cAMP to the culture medium. This agent (nilvadipine) was found to inhibit both sodium and calcium current on the cell membranes of differentiated NG108-15 cells. The inhibitory effect was reversible in a dose-dependent manner between 10 and 100 microM. A substantial washing-time with normal saline, over 20 minutes, was needed for complete recovery from inhibition. The higher the concentration of nilvadipine, the more suppressive was the action on the membrane potential. A higher dose of nilvadipine, 100 microM, caused the disappearance of the peak membrane potential. This effect appeared irreversible, when cells were incubated in the presence of 100 microM nilvadipine for a longer time. Nilvadipine may thus exert inhibitory effect on the electrophysiological activity of neuronal cells, especially the calcium current of the membrane.
采用电流钳法研究了尼伐地平对培养的神经母细胞瘤-胶质瘤杂交细胞NG108-15膜电位的影响。向培养基中添加1mM二丁酰环磷腺苷后，NG108-15细胞系可分化为神经元细胞。发现该药物（尼伐地平）可抑制分化的NG108-15细胞膜上的钠电流和钙电流。在10至100微摩尔之间，抑制作用呈剂量依赖性且可逆。需要用生理盐水大量冲洗20多分钟才能完全从抑制中恢复。尼伐地平浓度越高，对膜电位的抑制作用越强。较高剂量的尼伐地平（100微摩尔）导致膜电位峰值消失。当细胞在100微摩尔尼伐地平存在下孵育较长时间时，这种效应似乎是不可逆的。因此，尼伐地平可能对神经元细胞的电生理活性，尤其是膜的钙电流产生抑制作用。
