Tréfouël M J
C R Acad Hebd Seances Acad Sci D. 1976 Jan 5;282(1):123-5.
The study of the oxygen uptake by cultures of Sporothrix schenckii as measured with the Clark electrode has shown that when the fungus was grown in a liquid medium, the atmospheric oxygen went into solution very slowly even when the liquid was rapidly stirred. The partial oxygen pressure was very small after some days of culture (no more than 2 or 3% expressed as the saturated value). Hence, it is postulated that the linear part of the growth curve is due to the dissolved oxygen acting as a limiting factor. When the oxygen uptake by filaments, conidia, or yeasts isplotted against the time the curve variations follow the transformations of the fungus.
用克拉克电极测量申克孢子丝菌培养物对氧气的摄取研究表明,当真菌在液体培养基中生长时,即使液体快速搅拌,大气中的氧气溶解也非常缓慢。培养几天后,氧分压非常小(以饱和值表示不超过2%或3%)。因此,推测生长曲线的线性部分是由于溶解氧作为限制因素所致。当将菌丝、分生孢子或酵母对氧气的摄取量与时间作图时,曲线变化遵循真菌的转变过程。