Thieulant M L, Ducouret B, Samperez S, Jouan P
C R Acad Hebd Seances Acad Sci D. 1976 Jan 12;282(2):231-4.
When anterior hypophysis from male rats were incubated in the presence of tritiated 5 alpha-androstan-3 alpha, 17 beta-diol (3 alpha-diol), the major part of recovered pituitary radioactivity was constituted by 5 alpha-dihydrotestosterone (5 alpha-DHT). This steroid represented an average of 72% of total tissular radioactivity. In cytosol, the bound hormonal fraction was constituted by about 47% 5 alpha-DHT and 35% 3 alpha-diol, whereas free radioactivity contained 67% 5 alpha-DHT and 22% 3 alpha-diol. These results demonstrate an intensive conversion of 3 alpha-diol into 5 alpha-DHT and consequently that the transformation of 5 alpha-DHT into 3 alpha-diol is reversible. Thus it is reasonable to think that the biological action of 3 alpha-diol one can observe following in vivo or in vitro experiments is due, in fact, to its conversion into 5 alpha-DHT.
当将雄性大鼠的垂体前叶在含有氚标记的5α-雄甾烷-3α,17β-二醇（3α-二醇）的条件下孵育时，回收的垂体放射性的主要部分由5α-二氢睾酮（5α-DHT）构成。这种类固醇平均占总组织放射性的72%。在胞质溶胶中，结合的激素部分约由47%的5α-DHT和35%的3α-二醇构成，而游离放射性中含有67%的5α-DHT和22%的3α-二醇。这些结果表明3α-二醇能大量转化为5α-DHT，因此5α-DHT向3α-二醇的转化是可逆的。所以有理由认为，在体内或体外实验中观察到的3α-二醇的生物学作用，实际上是由于其转化为5α-DHT所致。
