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[通过亲和层析法从人血清中纯化胆碱酯酶]

[Purification of cholinesterase from human serum by affinity chromatography].

作者信息

Picard J

出版信息

C R Acad Hebd Seances Acad Sci D. 1976 Jan 12;282(2):235-7.

PMID:816547
Abstract

The human serum butyrylcholinesterase was rapidly purified with an affinity technique on meta-aminophenyltrimethylammonium-agarose; the ionic strength was 0.25 and the specific elution was achieved with 9-amino-10-methylacridinium. After ion exchange on DEAE-cellulose and molecular filtration, an enzyme purified more than ten thousand times was obtained.

摘要

用人血清丁酰胆碱酯酶在间氨基苯基三甲基氯化铵琼脂糖上采用亲和技术进行快速纯化;离子强度为0.25,用9-氨基-10-甲基吖啶进行特异性洗脱。经DEAE-纤维素离子交换和分子过滤后,获得了纯化程度超过一万倍的酶。

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