Pesce A J, Hsu A, Kornhauser C, Sethi K, Ooi B S, Pollak V E
Clin Chem. 1976 May;22(5):667-72.
We combined the use of a concentrating device (Minicon) and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate to semi-quantitate the concentration of (a) the collective low-molecular-weight proteins and (b) of albumin excreted in the urine of patients after renal transplantation. Analytical recovery of many serum proteins from samples concentrated 100-fold in the Minicon apparatus was about 70%. It was possible to examine many urine samples by polyacrylamide gel electrophoresis after concentration with this device. The reproducibility (CV) of the technique was on the order of 20% when albumin and low-molecular-weight protein were in about equal concentration. The method was adequate to differntiate glomerular and tubular proteinuria, because in glomerular proteinuria the ratio of albumin to low-molecular-weight proteins is about 20/1, whereas in tubular proteinuria the ratio is about 1/1.
我们联合使用一种浓缩装置（Minicon）和在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳，以半定量肾移植患者尿液中排泄的（a）低分子量蛋白质总和以及（b）白蛋白的浓度。从在Minicon装置中浓缩100倍的样品中对多种血清蛋白的分析回收率约为70%。使用该装置浓缩后，通过聚丙烯酰胺凝胶电泳检测多个尿液样品成为可能。当白蛋白和低分子量蛋白质浓度大致相等时，该技术的重现性（CV）约为20%。该方法足以区分肾小球性蛋白尿和肾小管性蛋白尿，因为在肾小球性蛋白尿中，白蛋白与低分子量蛋白质的比例约为20/1，而在肾小管性蛋白尿中该比例约为1/1。
