Yokoyama S, Kimura Y, Taketo M, Black J A, Ransom B R, Higashida H
Department of Biophysics, Neuroinformation Research Institute, Kanazawa University School of Medicine, Japan.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):634-41. doi: 10.1006/bbrc.1994.1495.
Two distinct cDNAs encoding bradykinin receptors (BKRs) were cloned from NG108-15 neuroblastoma-glioma hybrid cells. One was identical with rat uterus B2 BKR, whereas the other one (mBKR) had 91% amino acid homology to the rat B2 BKR and 82% homology to human B2 BKR. Southern blot analysis and genomic DNA cloning revealed that mBKR is derived from the mouse genome. The mBKR, expressed in Xenopus oocytes and COS-7 cells, produced functional BKRs that exhibited the properties of smooth muscle type B2 BKR. These results suggest that both the rat and mouse B2 BKRs of the smooth muscle type are expressed in NG108-15 cells.
从NG108 - 15神经母细胞瘤-胶质瘤杂交细胞中克隆出了两种编码缓激肽受体（BKRs）的不同cDNA。其中一种与大鼠子宫B2 BKR相同，而另一种（mBKR）与大鼠B2 BKR有91%的氨基酸同源性，与人类B2 BKR有82%的同源性。Southern印迹分析和基因组DNA克隆表明mBKR源自小鼠基因组。在非洲爪蟾卵母细胞和COS - 7细胞中表达的mBKR产生了具有平滑肌型B2 BKR特性的功能性BKRs。这些结果表明，平滑肌型的大鼠和小鼠B2 BKRs都在NG108 - 15细胞中表达。
