肾上腺髓质素刺激大鼠血管平滑肌细胞中环状AMP的形成。
Adrenomedullin stimulates cyclic AMP formation in rat vascular smooth muscle cells.
作者信息
Ishizaka Y, Ishizaka Y, Tanaka M, Kitamura K, Kangawa K, Minamino N, Matsuo H, Eto T
机构信息
First Department of Internal Medicine, Miyazaki Medical College.
出版信息
Biochem Biophys Res Commun. 1994 Apr 15;200(1):642-6. doi: 10.1006/bbrc.1994.1496.
A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 x 10(-9) M). In a receptor binding assay for AM in rat VSMC, the binding of [125I] AM was competitively inhibited by human AM, but not by human CGRP. Thus, AM is thought to increase intracellular cAMP in rat VSMC via its specific receptor to evoke vasodilation.
一种新发现的人类肽，肾上腺髓质素（AM）在大鼠血管平滑肌细胞（VSMC）中，以与人类降钙素基因相关肽（CGRP）相似的方式，呈剂量和时间依赖性地增加环磷酸腺苷（cAMP）水平。人AM的半数有效浓度（EC50）值为2×10⁻⁸ M，略高于CGRP的（8.5×10⁻⁹ M）。在大鼠VSMC中进行的AM受体结合试验中，[¹²⁵I]AM的结合被人AM竞争性抑制，但不被人CGRP抑制。因此，AM被认为通过其特异性受体增加大鼠VSMC中的细胞内cAMP，从而引起血管舒张。
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