Supplemental knowledge on survival of Thelohanellus kitauei spores in vitro.

In order to search the fate of Thelohanellus kitauei spores the extrusion rates of the polar filaments were monitored in vitro chronologically. Preserved spores suspended with various solutions at -70 degrees C showed almost the same vigorous pattern as early freezing stages up to 1,750 days after initial preservation. It revealed that the viabilities of some spores suspended with 0.45% and 0.9% NaCl solutions and distilled water at 5 degrees C continued for 1,628 days, 1,614 days and 1,721 days, respectively. And, the life spans of some spores in the previous solutions added with antibiotics at 5 degrees C were 1,628 days, 1,614 days and 1,714 days, respectively.