兔心脏保存后还原型巯基胺的强心作用

Cardiotonic effects of reduced sulfhydryl amines after preservation of rabbit hearts.

作者信息

Holdefer M M, Wicomb W N, Levy J V, Collins G M

机构信息

California Pacific Medical Center Research Institute, San Francisco 94115.

出版信息

J Heart Lung Transplant. 1994 Jan-Feb;13(1 Pt 1):157-9.

PMID:8167122

Abstract

This investigation was designed to examine the role of glutathione and other reduced sulfhydryl amines during reperfusion of the ischemic rabbit heart. To identify the biochemical features of reduced sulfhydryl amines contributing toward improved myocardial function, we investigated several chemical agents sharing a common property with glutathione (L-leucine, L-glycine, ascorbate, oxidized glutathione, L-cysteine). After a period of 24-hour hypothermic storage of the rabbit heart in a modified University of Wisconsin solution containing polyethylene glycol, the hearts were functionally evaluated on a Langendorff working heart model. The agents were then injected as a bolus (60 mumol/L) during reperfusion, and coronary flow and aortic output were measured. Control hearts were untreated. Reduced sulfhydryl amines (glutathione, L-cysteine) significantly improved coronary flow (p < 0.005) and cardiac output (p < 0.005). Ascorbate, L-leucine, L-glycine, and oxidized glutathione all failed to influence cardiac function.

摘要

本研究旨在探讨谷胱甘肽和其他还原型巯基胺在缺血兔心脏再灌注过程中的作用。为了确定有助于改善心肌功能的还原型巯基胺的生化特性，我们研究了几种与谷胱甘肽具有共同特性的化学试剂（L-亮氨酸、L-甘氨酸、抗坏血酸盐、氧化型谷胱甘肽、L-半胱氨酸）。在含有聚乙二醇的改良威斯康星大学溶液中对兔心脏进行24小时低温保存后，在Langendorff工作心脏模型上对心脏进行功能评估。然后在再灌注期间将这些试剂作为推注（60 μmol/L）注入，并测量冠状动脉流量和主动脉输出量。对照心脏未进行处理。还原型巯基胺（谷胱甘肽、L-半胱氨酸）显著改善了冠状动脉流量（p < 0.005）和心输出量（p < 0.005）。抗坏血酸盐、L-亮氨酸、L-甘氨酸和氧化型谷胱甘肽均未能影响心脏功能。