Weston K M, Alsalami M, Raison R L
Immunobiology Unit, School of Biological and Biomedical Science, University of Technology, Sydney, New South Wales, Australia.
Cytometry. 1994 Feb 1;15(2):141-7. doi: 10.1002/cyto.990150207.
The 90 degrees light scatter parameter of the flow cytometer was used to observe changes in the membrane of human lymphoblastoid cells (HMy2) as a result of the action of the cytolytic peptide, melittin. There was a rapid and concentration-dependent increase in 90 degrees light scatter after incubation of the cells with melittin, with the level of 90 degrees light scatter reaching a maximum after 2 min. Even after all the cells were killed, as determined by ethidium bromide incorporation, the 90 degrees light scatter continued to increase. Further, the 90 degrees light scatter changes were temperature dependent. The data are consistent with the formation of lipid vesicles, either attached to the cell membrane or intracellular, as confirmed by electron microscopy of cells treated with melittin. The results demonstrate the use of the flow cytometer to detect changes in the integrity of the cell membrane.
使用流式细胞仪的90度光散射参数来观察溶细胞肽蜂毒肽作用后人类淋巴母细胞（HMy2）细胞膜的变化。用蜂毒肽孵育细胞后，90度光散射迅速且呈浓度依赖性增加，2分钟后90度光散射水平达到最大值。即使通过溴化乙锭掺入确定所有细胞均已死亡，90度光散射仍继续增加。此外，90度光散射变化与温度有关。这些数据与通过用蜂毒肽处理的细胞的电子显微镜证实的附着于细胞膜或细胞内的脂质囊泡的形成一致。结果证明了使用流式细胞仪检测细胞膜完整性的变化。
