胸膜肺炎放线杆菌ApxIIA毒素的细胞毒性和溶血活性的差异表达
Differential expression of the cytotoxic and hemolytic activities of the ApxIIA toxin from Actinobacillus pleuropneumoniae.
作者信息
Tu A H, Hausler C, Young R, Struck D K
机构信息
Department of Medical Biochemistry and Genetics, Texas A&M Health Science Center, College of Medicine, College Station 77843-1114.
出版信息
Infect Immun. 1994 May;62(5):2119-21. doi: 10.1128/iai.62.5.2119-2121.1994.
Abstract
The ApxIIA protein secreted from Actinobacillus pleuropneumoniae is both hemolytic and cytotoxic. However, when the cloned apxII operon is expressed in Escherichia coli, two forms of the ApxIIA protein can be recovered. Toxin which remains intracellular has hemolytic and cytotoxic activities, while toxin that is secreted is cytotoxic with little or no hemolytic activity. This indicates that the cytotoxicity of ApxIIA is independent of its hemolytic activity.
摘要
胸膜肺炎放线杆菌分泌的ApxIIA蛋白具有溶血活性和细胞毒性。然而，当克隆的apxII操纵子在大肠杆菌中表达时，可以获得两种形式的ApxIIA蛋白。保留在细胞内的毒素具有溶血活性和细胞毒性，而分泌的毒素具有细胞毒性，但溶血活性很小或没有。这表明ApxIIA的细胞毒性与其溶血活性无关。
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