Shannon W A, Wasserkrug H L, Seligman A M
J Histochem Cytochem. 1976 Mar;24(3):540-55. doi: 10.1177/24.3.816966.
The oxidation of p-N,N-dimethylamino-beta-phenethylamine (DAPA) by amine oxidase(s) (AO), i.e., diamine oxidase (DAO), monoamine oxidase (MAO) and/or possibly other oxidases, has been previously demonstrated. This study reports the results of variations in fixation procedures and the incorporation of a series of possible "activators" and inhibitors into the DAPA oxidase (DAPAO) and DAPAO-BSPT [2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl) tetrazolium chloride] reaction media in an attempt to elucidate the oxidase(s) involved. Results of these studies are indicative of at least two different oxidases acting preferentially on one or the other of the two systems. The presence of MAO, especially in unfixed tissue, and DAO, especially in fixed tissue, is denoted and that of other oxidase(s) is connoted.
先前已证实，胺氧化酶（AO），即二胺氧化酶（DAO）、单胺氧化酶（MAO）和/或可能的其他氧化酶可氧化对-N，N-二甲基氨基-β-苯乙胺（DAPA）。本研究报告了固定程序变化以及在DAPA氧化酶（DAPAO）和DAPAO-BSPT [2-（2'-苯并噻唑基）-5-苯乙烯基-3-（4'-邻苯二甲酰肼基）四唑氯化物]反应介质中加入一系列可能的“激活剂”和抑制剂的结果，以试图阐明所涉及的氧化酶。这些研究结果表明至少有两种不同的氧化酶优先作用于这两个系统中的一个或另一个。表明存在MAO，特别是在未固定组织中，以及DAO，特别是在固定组织中，并暗示存在其他氧化酶。
