Arai Y, Enomoto H, Ito K, Furukawa M, Kubota A, Tsukuda M
Department of Head and Neck, Kanagawa Cancer Center, Yokohama.
Nihon Jibiinkoka Gakkai Kaiho. 1994 Feb;97(2):254-9. doi: 10.3950/jibiinkoka.97.254.
The DNA ploidy of fresh frozen tissues from head and neck squamous cell carcinomas was determined by flow cytometry, with the aim of investigating whether DNA ploidy correlates with various clinical and pathological parameters. The subjects were 51 patients who had undergone radical surgery in our department. The DNA ploidy pattern was classified into three types, diploid, single aneuploid and multiploid, according to the DNA index and the DNA histogram. This is our original classification. No particular correlation could be detected between the DNA ploidy pattern and sex, age, primary tumor site or disease stage. The degree of tissue differentiation tended to be poorer in aneuploid than in diploid tissues. The efficacy of chemotherapy was higher in aneuploid than in diploid cases. The recurrence rate was significantly lower in diploid than in multiploid cases. When disease stage, degree of histological differentiation, the efficacy of chemotherapy and the DNA ploidy pattern were subjected to multivariate analysis for correlation with prognosis, the DNA ploidy pattern showed the highest correlation. Our results suggest that the DNA ploidy, as analyzed by flow cytometry, can serve as useful prognostic factor.
采用流式细胞术测定头颈部鳞状细胞癌新鲜冷冻组织的DNA倍体，目的是研究DNA倍体是否与各种临床和病理参数相关。研究对象为51例在我科接受根治性手术的患者。根据DNA指数和DNA直方图，将DNA倍体模式分为二倍体、单非整倍体和多倍体三种类型。这是我们最初的分类。未发现DNA倍体模式与性别、年龄、原发肿瘤部位或疾病分期之间存在特定相关性。非整倍体组织的组织分化程度往往比二倍体组织差。非整倍体病例的化疗疗效高于二倍体病例。二倍体病例的复发率显著低于多倍体病例。当对疾病分期、组织学分化程度、化疗疗效和DNA倍体模式进行多因素分析以探讨与预后的相关性时，DNA倍体模式显示出最高的相关性。我们的结果表明，通过流式细胞术分析的DNA倍体可作为有用的预后因素。
