Besson I, Marín P, Merino A, Urbano-Ispízua A, Rozman C, Vives Corrons J L
Servei d'Hematologia Biològica y, Hospital Clínic i Provincial de Barcelona, Universitat de Barcelona.
Sangre (Barc). 1993 Dec;38(6):481-4.
The efficiency of the GIBCORkit "Human Bone Marrow Stem Cell Proliferation Kit" for haemopoietic progenitors cultures, has been assessed in 24 bone marrow samples. The results, compared with those obtained in a parallel study with the routine method used in our laboratory (reference method), suggests that the kit has higher capacity for detecting haemopoietic progenitors, which is due to the increased number of BFU-E (108 +/- 78 vs 23 +/- 23, p < 0.0001). Total CFU-GM (considered here as the sum of CFU-G and CFU-M), do not differ significantly from the reference method (70 +/- 64 vs 77 +/- 64, p = 0.60) despite its increased CFU-G growth (25 +/- 26 vs 67 +/- 54, p < 0.0001). The finding of similar results in a multicentric study may contribute to demonstrate the advantages of this standardized method in the clinical practice.
已在24份骨髓样本中评估了GIBCORkit“人骨髓干细胞增殖试剂盒”用于造血祖细胞培养的效率。将结果与我们实验室采用的常规方法（参考方法）在一项平行研究中获得的结果进行比较，结果表明该试剂盒检测造血祖细胞的能力更强，这是由于爆式红系集落形成单位（BFU-E）数量增加（108±78对23±23，p<0.0001）。尽管粒系集落形成单位（CFU-G）生长增加（25±26对67±54，p<0.0001），但总粒-巨噬系集落形成单位（此处视为CFU-G和CFU-M之和）与参考方法相比无显著差异（70±64对77±64，p = 0.60）。在一项多中心研究中发现类似结果，可能有助于证明这种标准化方法在临床实践中的优势。
