Wdzieczak-Bakala J, Grillon C, Robinson S, Riches A, Carde P, Lenfant M
Institut de chimie des substances naturelles, Centre national de la recherche scientifique, Gif-sur-Yvette, France.
Bull Cancer. 1993 May;80(5):391-6.
The comparative degradation of N-Ac-Ser-Asp-Lys-Pro (AcSDKP), a negative regulator controlling the proliferation of the hematopoietic pluripotent stem cell, was investigated following incubation with plasma, bone marrow and spleen cells from normal mice and mice bearing a transplantable myeloid leukemia. Using the tetrapeptide, specifically radiolabelled in the lysyl residue, degradation of [3H]AcSDKP was followed by measurement of [3H]Lys formation resulting from its catabolism. It was shown that already after 1 h the degradation of AcSDKP in plasma from leukemic mice was higher compared to that following incubation in plasma from normal mice, whereas incubation with bone marrow cells exhibits a small difference only after 4 hours incubation. However, no increase of AcSDKP catabolic activity was observed following incubation with spleen cells from leukemic animals when compared with incubation of normal spleen cells.
对造血多能干细胞增殖起负调控作用的N-乙酰丝氨酰-天冬氨酰-赖氨酰-脯氨酸（AcSDKP），在与正常小鼠及患有可移植性髓系白血病小鼠的血浆、骨髓和脾细胞共同孵育后，对其降解情况进行了比较研究。使用在赖氨酰残基上特异性放射性标记的四肽，通过测量[3H]AcSDKP分解代谢产生的[3H]赖氨酸的形成情况来跟踪[3H]AcSDKP的降解。结果表明，与在正常小鼠血浆中孵育相比，在白血病小鼠血浆中孵育1小时后，AcSDKP的降解就更高，而与骨髓细胞共同孵育时，仅在孵育4小时后才表现出微小差异。然而，与正常脾细胞孵育相比，白血病动物脾细胞孵育后未观察到AcSDKP分解代谢活性增加。
