Sattler C A, Staehelin L A
Tissue Cell. 1976;8(1):1-18. doi: 10.1016/0040-8166(76)90016-1.
Reconstruction of the oral cavity from 17 0.5 mum thick serial sections observed with a high voltage electron microscope (JEM-1000, operated at 1000 kV) has enabled us to describe the in situ shape of the cavity, the orientation of the membranelles, oral ribs, cytostomal lip and forming food vacuole of Tetrahymena pyriformis. The study also showed that many different sets of microtubules encircle the oral cavity forming an interwoven, basket-like structure around the cavity thus providing it with considerable structural rigidity. By correlating results obtained from the reconstruction with results obtained from scanning electron microscopy and freeze-fracturing we have been able to elucidate the probable mechanism of how food particles are propelled into and through the oral cavity.
利用高压电子显微镜（JEM - 1000，在1000 kV下运行）观察17个0.5微米厚的连续切片，对梨形四膜虫口腔进行重建，使我们能够描述其口腔的原位形态、膜状器、口肋、胞口唇以及正在形成的食物泡的方向。该研究还表明，许多不同组的微管环绕口腔，在口腔周围形成一个交织的、篮状结构，从而赋予其相当大的结构刚性。通过将重建结果与扫描电子显微镜和冷冻断裂结果相关联，我们得以阐明食物颗粒被推进口腔并穿过口腔的可能机制。
