人完整胰岛素原的免疫放射分析在2型糖尿病、糖耐量受损和高雄激素血症患者中的应用。

Immunoradiometric assay of human intact proinsulin applied to patients with type 2 diabetes, impaired glucose tolerance, and hyperandrogenism.

作者信息

Chevenne D, Ruiz J, Lohmann L, Laudat A, Leblanc H, Gray I P, Passa P, Porquet D

机构信息

Hôpital Robert Debré, Laboratoire de Biochimie-Hormonologie, Paris, France.

出版信息

Clin Chem. 1994 May;40(5):754-7.

PMID:8174247

Abstract

We describe an immunoradiometric assay for human intact proinsulin in serum. In this method, one monoclonal antibody, coated onto polyacrylamide beads, cross-reacts with proinsulins and insulin. A sandwich is formed with intact proinsulin, split (65-66) proinsulin, and des (64-65) proinsulin binding with an 125I-labeled monoclonal antibody specific for an epitope at the intact B-C junction of proinsulin. Because split (65-66) and des (64-65) proinsulin concentrations are very low in serum, this assay essentially measures intact proinsulin. When we used 1-mL serum samples, the mean detection limit was 0.4 pmol/L. Mean proinsulin concentrations (pmol/L) were 3.4 (range 1-9.1) in healthy fasting subjects, 28.5 (9.7-101) in patients with type 2 diabetes (treated with metformin and sulfonylureas), 5.0 (1.6-9.3) in women with hyperandrogenism and normal insulinemia, 10.3 (2.6-36) in women with hyperandrogenism and hyperinsulinemia, and 8.5 (4.8-21.3) in patients with impaired glucose tolerance.

摘要

我们描述了一种用于检测血清中完整人胰岛素原的免疫放射分析方法。在该方法中，一种包被在聚丙烯酰胺珠上的单克隆抗体与胰岛素原和胰岛素发生交叉反应。完整胰岛素原、裂解（65-66）胰岛素原和缺失（64-65）胰岛素原与一种针对胰岛素原完整B-C连接位点表位的125I标记单克隆抗体结合形成夹心结构。由于血清中裂解（65-66）和缺失（64-65）胰岛素原的浓度非常低，该分析方法主要检测完整胰岛素原。当我们使用1 mL血清样本时，平均检测限为0.4 pmol/L。健康空腹受试者的胰岛素原平均浓度（pmol/L）为3.4（范围1-9.1），2型糖尿病患者（接受二甲双胍和磺脲类药物治疗）为28.5（9.7-101），高雄激素血症且胰岛素血症正常的女性为5.0（1.6-9.3），高雄激素血症且高胰岛素血症的女性为10.3（2.6-36），糖耐量受损患者为8.5（4.8-21.3）。