Zhang J Z
Department of Pathology, Third Military Medical College, Chongqing.
Zhonghua Zhong Liu Za Zhi. 1993 Jul;15(4):259-63.
Twenty-seven cases of non-Hodgkin's lymphoma (NHL) were studied by Southern blot to analyse gene rearrangements of immunoglobulin heavy chain (IgH), kappa chain (Igk) and T-cell receptor beta (T beta). Genomic DNA extraction from tissues was modified by increasing concentration of proteinase k and SDS in the extraction buffer and prolongation of incubation time. The results showed that 26 of 27 cases (96%) presented gene rearrangements of immunoglobulin and T-cell receptor of NHLs with T-cell phenotype, 11 of 13 cases showed T beta gene rearrangement. All the B-cell lymphomas analysed displayed IgH and Igk gene rearrangements. Reactive lymphoid hyperplasia demonstrated germline configuration of the above mentioned genes. These results suggest that gene rearrangement analysis may considerably help in determining the clonality and lineage of lymphomas, and distinguish benign reactive lymphoia hyperplasia from malignant lymphoma.
对27例非霍奇金淋巴瘤（NHL）进行了Southern印迹分析，以检测免疫球蛋白重链（IgH）、κ链（Igk）和T细胞受体β（Tβ）的基因重排。通过提高提取缓冲液中蛋白酶K和SDS的浓度并延长孵育时间，对从组织中提取基因组DNA的方法进行了改进。结果显示，27例中有26例（96%）呈现出具有T细胞表型的NHL免疫球蛋白和T细胞受体的基因重排，13例中有11例显示Tβ基因重排。所有分析的B细胞淋巴瘤均显示IgH和Igk基因重排。反应性淋巴组织增生显示上述基因的种系构型。这些结果表明，基因重排分析可能对确定淋巴瘤的克隆性和谱系有很大帮助，并能将良性反应性淋巴细胞增生与恶性淋巴瘤区分开来。
