[用合成探针检测P388/ADR和L1210/DDP耐药株中谷胱甘肽S转移酶π信使核糖核酸的表达]
[The expression of GST pi mRNA in the P388/ADR and L1210/DDP resistant lines examined by synthetic probe].
作者信息
Liu Y B
机构信息
Department of Clinical Pharmacology, North Taiping Road Hospital, Academy of Military Medical Sciences, Beijing.
出版信息
Zhonghua Zhong Liu Za Zhi. 1993 Jul;15(4):264-7.
We examined the expression of GST pi mRNA in the P388/ADR and L1210/DDP resistant lines by using alpha-32p-labeled synthetic 45 bp for an anionic glutathione transferase (GST pi). The dot blot results showed that GST pi mRNA increased significantly (P < 0.01) by 1.28 and 1.57-fold in ADR-resistant cells (ADR resistant 23.6 and 44.4-fold) compared to sensitive cell line, whereas the L1210/DDP increased by 1.17-fold (P < 0.05). Further evidence of the expression of GST pi mRNA provided by Northern blot analysis indicated 1.28 and 1.41-fold increase in ADR resistant lines (resistant 23.6 and 44.4 fold). While Northern blot showed a 0.75 KB GST pi mRNA. Southern blot indicated that GST pi gene was represented by fragments of approximately 6.0 and 5.1 kb after DNA was digested with Hind III. Verapamil increased the expression of GST pi mRNA in P388/ADR adriamycin resistant lines.
我们使用α-32P标记的针对阴离子谷胱甘肽转移酶（GST pi）的45 bp合成探针，检测了P388/ADR和L1210/DDP耐药细胞系中GST pi mRNA的表达。斑点印迹结果显示，与敏感细胞系相比，ADR耐药细胞中GST pi mRNA显著增加（P < 0.01），增加了1.28倍和1.57倍（ADR耐药分别为23.6倍和44.4倍），而L1210/DDP增加了1.17倍（P < 0.05）。Northern印迹分析提供的GST pi mRNA表达的进一步证据表明，ADR耐药细胞系中增加了1.28倍和1.41倍（耐药分别为23.6倍和44.4倍）。同时，Northern印迹显示有一条0.75 KB的GST pi mRNA。Southern印迹表明，用Hind III消化DNA后，GST pi基因由约6.0和5.1 kb的片段代表。维拉帕米增加了P388/ADR阿霉素耐药细胞系中GST pi mRNA的表达。
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