Jacob J R, Liu R H, Roneker C A, de Noronha F, Hotchkiss J H, Tennant B C
College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Exp Cell Res. 1994 May;212(1):42-8. doi: 10.1006/excr.1994.1116.
Primary woodchuck (Marmota monax) hepatocytes from normal woodchucks and woodchucks with chronic woodchuck hepatitis virus (WHV) infection were cultured in either a conventional serum-containing medium or a serum-free medium. The de novo synthesis of the plasma proteins albumin, transferrin, fibrinogen, and complement C3 were identical under both conditions. However, expression of the WHV and the synthesis of nitric oxide were diminished under serum-free conditions. Primary woodchuck hepatocytes cultured in conventional, serum-containing medium were immortalized utilizing the simian virus 40 T antigen oncogene. Immortalized hepatic cell lines retained differentiated functions of nitric oxide synthesis and expression of complement C3. The woodchuck hepatocyte culture model will supplement current experimental methods, allowing investigation of hepadnaviral pathogenesis, including hepatocarcinogenesis in vitro.
将来自正常土拨鼠和感染慢性土拨鼠肝炎病毒（WHV）的土拨鼠的原代土拨鼠（Marmota monax）肝细胞，在传统的含血清培养基或无血清培养基中培养。在这两种条件下，血浆蛋白白蛋白、转铁蛋白、纤维蛋白原和补体C3的从头合成是相同的。然而，在无血清条件下，WHV的表达和一氧化氮的合成减少。利用猿猴病毒40 T抗原癌基因，将在传统的含血清培养基中培养的原代土拨鼠肝细胞永生化。永生化肝细胞系保留了一氧化氮合成和补体C3表达的分化功能。土拨鼠肝细胞培养模型将补充当前的实验方法，允许在体外研究嗜肝DNA病毒发病机制，包括肝癌发生。
