Konrad L, Becker G, Schmidt A, Klöckner T, Kaufer-Stillger G, Dreschers S, Edström J E, Gateff E
Institut für Genetik, Johannes Gutenberg Universität, Mainz, Federal Republic of Germany.
Dev Biol. 1994 May;163(1):98-111. doi: 10.1006/dbio.1994.1126.
The tumor suppressor gene, lethal(3)malignant blood neoplasm-1+, of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1+ gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed.
果蝇的肿瘤抑制基因致死（3）恶性血液肿瘤-1+是吞噬血细胞类型即浆细胞分化所必需的。在纯合突变状态下，它会导致这些血细胞发生恶性转化。我们在此展示了l（3）mbn-1+基因在发育过程中的克隆、测序、结构及表达情况。通过种系转化、回复体的产生以及在血细胞和其他组织中检测相应的mRNA来鉴定克隆的基因。发现推定的MBN83蛋白富含G-S的C末端与人细胞角蛋白K1、K10以及小鼠loricrin具有同源性。文中讨论了野生型l（3）mbn-1+基因的结构和可能的功能。
