von Allmen E, Rieben R, Nydegger U E
Regional Red Cross Blood Transfusion Center, University of Berne, Switzerland.
J Immunol Methods. 1994 May 2;171(1):85-92. doi: 10.1016/0022-1759(94)90231-3.
Little is known about the correlation between the amount of anti-A antibodies and the respective complement activating capacity. We have therefore developed a new ELISA procedure for the study of complement activation by anti-A antibodies bound to immobilized blood group substance A through the classical pathway. C1q binding capacity of anti-A was then compared to both anti-A immunoglobulin (Ig) isotype serum concentrations and to the hemolysing capacity of O type sera. We have demonstrated that sera with low anti-A IgG and IgM levels produced low C1q binding activity and no hemolysis of human A1 red blood cells (RBC), whereas higher anti-A Ig levels resulted in either weak or strong C1q binding capacity as well as weak or strong RBC hemolysis. A correlation of rs = 0.755 was observed between C1q-ABO-ELISA and hemolysin assay results, although only 58% of the O type sera containing C1q binding anti-A lysed A1 type RBC under the used conditions. The C1q-ABO-ELISA is therefore a more sensitive assay for detecting 'dangerous' O type donors for blood transfusion to A type recipients than screening tests for hemolytic anti-A or determination of anti-A Ig levels alone.
关于抗A抗体量与各自补体激活能力之间的相关性，目前所知甚少。因此，我们开发了一种新的酶联免疫吸附测定（ELISA）方法，用于研究通过经典途径与固定化血型物质A结合的抗A抗体所引发的补体激活。然后将抗A的C1q结合能力与抗A免疫球蛋白（Ig）同种型血清浓度以及O型血清的溶血能力进行比较。我们已经证明，抗A IgG和IgM水平较低的血清产生的C1q结合活性较低，并且不会使人A1红细胞（RBC）发生溶血，而较高的抗A Ig水平则导致C1q结合能力弱或强，以及RBC溶血弱或强。尽管在所用条件下，只有58%含有C1q结合抗A的O型血清能使A1型RBC发生溶血，但在C1q-ABO-ELISA与溶血素测定结果之间观察到rs = 0.755的相关性。因此，对于检测向A型受血者输血的“危险”O型献血者而言，C1q-ABO-ELISA是一种比单独的抗A溶血筛查试验或抗A Ig水平测定更为灵敏的检测方法。
