Kobayashi H, Oishi S, Koumoto A, Yoshida T, Matsunaga T, Ogawa M
First Department of Internal Medicine, Kyourin University School of Medicine.
Kansenshogaku Zasshi. 1994 Mar;68(3):314-8. doi: 10.11150/kansenshogakuzasshi1970.68.314.
The anti-endotoxin activity of mouse monoclonal IgM antibody E5 was determined against various lipopolysaccharides (LPSs) derived from Escherichia coli J5, E. coli O111:B4, Klebsiella pneumoniae and Pseudomonas aeruginosa by measuring limulus activity by endospecy method. In general, above 0.2 microgram/ml of E5 reduced almost dose-dependently the limulus activities of all LPSs tested, although the degree of reduction in limulus activity somewhat varied among bacterial species. The limulus activities of LPS from E. coli J5, E. coli O111:B4, K. pneumoniae and P. aeruginosa decreased by 72%, 50%, 44%, and 58%, respectively, by exposure to 5.0 micrograms/ml of E5 for 2 hrs.
通过内毒素特异性鲎试剂法测量鲎试剂活性，测定了小鼠单克隆IgM抗体E5对源自大肠杆菌J5、大肠杆菌O111:B4、肺炎克雷伯菌和铜绿假单胞菌的各种脂多糖（LPS）的抗内毒素活性。一般来说，E5浓度高于0.2微克/毫升时，几乎呈剂量依赖性地降低了所有测试LPS的鲎试剂活性，尽管不同细菌种类的鲎试剂活性降低程度有所不同。将大肠杆菌J5、大肠杆菌O111:B4、肺炎克雷伯菌和铜绿假单胞菌的LPS分别暴露于5.0微克/毫升的E5中2小时后，其鲎试剂活性分别降低了72%、50%、44%和58%。
