Parton R, Hall E, Wardlaw A C
Department of Microbiology, University of Glasgow.
J Med Microbiol. 1994 May;40(5):307-12. doi: 10.1099/00222615-40-5-307.
Phase I strains 18-323, Tohama and L-84 of Bordetella pertussis produced paroxysmal coughing when encased in agarose beads and administered intrabronchially to adult Sprague-Dawley rats. In contrast, the Phase IV variant of strain L-84 was inactive in cough induction, as was strain BP 357, a transposon-insertion mutant which is deficient only in pertussis toxin (PT). Strain BPM 1809, which lacks only the heat-labile toxin, was similar to the unmodified Phase I strains for cough induction, indicating that this toxin is not needed to induce coughing. B. parapertussis also was inactive as a cough inducer. These results indicate that PT, present in Phase I strains of B. pertussis, and absent from Phase IV strains, strain BP 357 and B. parapertussis, is essential for the induction of paroxysmal coughing in this rat model of whooping cough. Prior injection of DTP (whole-cell) vaccine greatly reduced the incidence of coughing in rats challenged subsequently with Phase I B. pertussis. Serological responses were monitored after intrabronchial infection with the various bacterial strains and after vaccination and challenge. The PT-positive or -negative status of the strains in vivo was confirmed by the appropriate presence or absence of anti-PT IgG in the convalescent sera.
百日咳博德特氏菌的I期菌株18 - 323、托哈马菌株和L - 84，当包裹在琼脂糖珠中并经支气管内给予成年斯普拉格-道利大鼠时，会引起阵发性咳嗽。相比之下，L - 84菌株的IV期变体在诱导咳嗽方面无活性，仅缺乏百日咳毒素（PT）的转座子插入突变体BP 357菌株也是如此。仅缺乏不耐热毒素的BPM 1809菌株在诱导咳嗽方面与未修饰的I期菌株相似，表明诱导咳嗽不需要这种毒素。副百日咳博德特氏菌作为咳嗽诱导剂也无活性。这些结果表明，百日咳博德特氏菌I期菌株中存在而IV期菌株、BP 357菌株和副百日咳博德特氏菌中不存在的PT，在这种百日咳大鼠模型中对于诱导阵发性咳嗽至关重要。预先注射DTP（全细胞）疫苗可大大降低随后用I期百日咳博德特氏菌攻击的大鼠的咳嗽发生率。在用各种细菌菌株进行支气管内感染后以及在疫苗接种和攻击后监测血清学反应。通过恢复期血清中抗PT IgG的适当存在或不存在，证实了体内菌株的PT阳性或阴性状态。
