Giα1及其处于结合GTP和GDP状态的突变体的结晶与初步晶体学研究。
Crystallization and preliminary crystallographic studies of Gi alpha 1 and mutants of Gi alpha 1 in the GTP and GDP-bound states.
作者信息
Coleman D E, Lee E, Mixon M B, Linder M E, Berghuis A M, Gilman A G, Sprang S R
机构信息
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050.
出版信息
J Mol Biol. 1994 May 13;238(4):630-4. doi: 10.1006/jmbi.1994.1320.
Several different crystal forms of Gi alpha 1 have been grown and analyzed. Crystals of native protein containing bound GTP gamma S belong to space group P3(1)2(1) or P3(2)2(1) with cell dimensions a,b = 80.6 A and c = 106.3 A and diffract to a resolution of 1.9 A using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a,b = 121.3 A, and c = 67.7 A and diffract to 3.0 A. Data sets from crystals grown using mutant proteins have also been obtained and characterized.
已培养并分析了几种不同晶体形式的Giα1。含有结合GTPγS的天然蛋白质晶体属于空间群P3(1)2(1)或P3(2)2(1)，晶胞参数a、b = 80.6 Å，c = 106.3 Å，使用同步辐射可衍射至1.9 Å的分辨率。含有结合GDP的天然蛋白质晶体属于空间群I4，晶胞参数a、b = 121.3 Å，c = 67.7 Å，可衍射至3.0 Å。还获得并表征了使用突变蛋白培养的晶体的数据集。
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