使用傅里叶变换红外显微光谱法检测碳氧血红蛋白。
Detection of carboxyhemoglobin using Fourier transform infrared microspectroscopy.
作者信息
Mori A, Shioda T, Takeda E, Genma M, Suzuki H, Ishiyama I
机构信息
Department of Legal Medicine, School of Medicine, Teikyo University.
出版信息
Nihon Hoigaku Zasshi. 1994 Feb;48(1):1-6.
We investigated the absorption of carboxyhemoglobin (COHb) using a Fourier transform infrared (FTIR) microscopy system. Weak absorption at 1969 cm-1 due to Fe<--CO and strong absorption due to N-H stretching vibration, > C = O stretching vibration and -NH bending vibration (1544 cm-1) were observed. The ratio of absorbance at 1969 cm-1/1544 cm-1 can be used as an index of CO saturation. Therefore, when formalin-fixed blood remains in blood vessels in formalin-fixed tissues, the amount of COHb can be calculated using this FTIR microscopy system.
我们使用傅里叶变换红外（FTIR）显微镜系统研究了碳氧血红蛋白（COHb）的吸收情况。观察到由于Fe<--CO导致的在1969 cm-1处的弱吸收以及由于N-H伸缩振动、>C = O伸缩振动和-NH弯曲振动（1544 cm-1）导致的强吸收。1969 cm-1/1544 cm-1处的吸光度比值可作为CO饱和度的指标。因此，当福尔马林固定组织中的血管内留存有福尔马林固定的血液时，可使用该FTIR显微镜系统计算COHb的含量。
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