[志贺菌地高辛标记virG DNA探针的制备及其同源性分析]

[Preparation of digoxigenin labeled virG DNA probe of Shigella and its homology analysis].

作者信息

She F, Bao Y

机构信息

Laboratory of Genetic Engineering, Fujian Medical College, Fuzhou.

出版信息

Wei Sheng Wu Xue Bao. 1993 Oct;33(5):368-73.

PMID:8178513

Abstract

Using hapten digoxigenin labeled 1. 4kb virG DNA probe of S. flexneri 2a YSH6000. We examined the DNA homology among 73 strains of Shigella, 32 strains of EIEC, 17 strains of Yersinia, 5 strains of S. typhi and 5 strains of E. coli. At the same time strains of Shigella, EIEC and Yesinia were tested by Sereny assay. We concluded that virG DNA is conserved both in Shigella and EIEC and is related to Sereny test only in Shigella and EIEC but not in Yesinia. The coincidence rate of hybridization and Sereny test was 87.5% and 90.9% for S. sonni and EIEC respectively.

摘要

使用地高辛配基标记的福氏志贺菌2a YSH6000 1.4kb virG DNA探针，我们检测了73株志贺菌、32株肠侵袭性大肠埃希菌（EIEC）、17株耶尔森菌、5株伤寒沙门菌和5株大肠埃希菌之间的DNA同源性。同时，对志贺菌、EIEC和耶尔森菌菌株进行了Sereny试验。我们得出结论，virG DNA在志贺菌和EIEC中均保守，且仅在志贺菌和EIEC中与Sereny试验相关，而在耶尔森菌中不相关。宋内志贺菌和EIEC的杂交与Sereny试验的符合率分别为87.5%和90.9%。