Qiao L, Tian Y, Mang K
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1993 Oct;33(5):383-6.
Two crystal protein genes, the 5.3kb and 6.6kb class respectively, from Bacillus thuringiensis subsp. kurstaki HD-1 (B. t HD-1) had been cloned previously. Based on the classification system of Hofte and Whiteley, these two genes should belong to Cry I A (b) and Cry I A (c) gene type respectively. The nucleotide sequence of the toxic domain of this Cry I A (c) gene from B. t kurstaki HD-1 is firstly reported here and compared with that of Cry I A (c) gene from B. t HD-73 and Cry I A (b) gene from B. t HD-1.
先前已克隆了来自苏云金芽孢杆菌库斯塔克亚种HD-1(B. t HD-1)的两个晶体蛋白基因,分别为5.3kb和6.6kb类别。根据霍夫特和怀特利的分类系统,这两个基因应分别属于Cry I A (b)和Cry I A (c)基因类型。本文首次报道了来自B. t库斯塔克HD-1的该Cry I A (c)基因毒性结构域的核苷酸序列,并将其与来自B. t HD-73的Cry I A (c)基因和来自B. t HD-1的Cry I A (b)基因的核苷酸序列进行了比较。