Yanoff M
Doc Ophthalmol. 1976 Apr 28;41(1):157-204. doi: 10.1007/BF00144715.
Four main areas were explored: 1) the proper medium for culturing corneal tissue; 2) the effect of serum on tissue growth in vitro; 3) the interrelationships in vitro between corneal epithelium and endothelium, and 4) the biology of cultures of whole corneas (organ cultures). Modified Eagle's minimal essential medium (MEM) proved to be an excellent culture fluid. Corneal tissue could be grown in MEM without serum or clot, thus providing a defined culture medium. The biology in vitro of outgrowths of multilayered corneal epithelium and monolayered corneal endothelium are discussed. Contact inhibition between epithelium and endothelium is demonstrated in whole corneal (organ) cultures.
1）培养角膜组织的合适培养基；2）血清对体外组织生长的影响；3）角膜上皮和内皮在体外的相互关系，以及4）全角膜培养物（器官培养）的生物学特性。改良的伊格尔最低必需培养基（MEM）被证明是一种出色的培养液。角膜组织可以在不含血清或凝块的MEM中生长，从而提供一种明确的培养基。讨论了多层角膜上皮和单层角膜内皮生长物的体外生物学特性。在全角膜（器官）培养中证明了上皮和内皮之间的接触抑制。
