Opara E C, Garfinkel M, Hubbard V S, Burch W M, Akwari O E
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Am J Physiol. 1994 Apr;266(4 Pt 1):E635-9. doi: 10.1152/ajpendo.1994.266.4.E635.
The purpose of the present study was to examine the role played by structural differences among fatty acids in their effect on insulin secretion by isolated perifused murine islets. Insulin secretion measured by radioimmunoassay was assessed either as total insulin output (ng.6 islets-1.20 min-1) or as percent of basal insulin secretion. Raising the glucose concentration from a basal 5.5 to 27.7 mM caused an increase of insulin output from 6.69 +/- 1.59 to 19.92 +/- 4.99 ng.6 islets-1.20 min-1 (P < 0.05) in control (untreated) islets. However, after 20-min exposure of islets to 5 mM 16:0 or 18:2, the effect of 27.7 mM glucose was enhanced or diminished, respectively. Basal insulin output (100% basal) changed to 44 +/- 10% basal (P < 0.005) with the addition of 5 mM 4:0 but was not altered when 4:0 was replaced by 6:0. Insulin output increased modestly with 5 mM 8:0 but significantly (P < 0.05) with 10:0 until a maximal of 280 +/- 24% basal with 12:0 (P < 0.01), then fell to 110 +/- 18 and 93 +/- 15% basal (P < 0.05) with 14:0 and 16:0, respectively. The addition of 5 mM 18:0 inhibited insulin secretion to 30 +/- 10% of basal (P < 0.003), and this effect was not caused by fatty acid interference with insulin assay.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是检验脂肪酸结构差异在其对分离的经外周灌注的小鼠胰岛胰岛素分泌的影响中所起的作用。通过放射免疫测定法测量的胰岛素分泌，以总胰岛素输出量（纳克·6个胰岛⁻¹·20分钟⁻¹）或基础胰岛素分泌的百分比来评估。将葡萄糖浓度从基础的5.5毫摩尔/升提高到27.7毫摩尔/升，导致对照（未处理）胰岛的胰岛素输出量从6.69±1.59增加到19.92±4.99纳克·6个胰岛⁻¹·20分钟⁻¹（P<0.05）。然而，胰岛在5毫摩尔16:0或18:2中暴露20分钟后，27.7毫摩尔葡萄糖的作用分别增强或减弱。添加5毫摩尔4:0时，基础胰岛素输出量（100%基础值）变为44±10%基础值（P<0.005），但用6:0替代4:0时则未改变。5毫摩尔8:0时胰岛素输出量适度增加，而用10:0时则显著增加（P<0.05），直到12:0时达到基础值的280±24%（P<0.01），然后分别降至14:0和16:0时的110±18%和93±
