培养星形胶质细胞中甲状旁腺激素/甲状旁腺激素相关肽受体的鉴定与特性分析
Identification and characterization of parathyroid hormone/parathyroid hormone-related peptide receptor in cultured astrocytes.
作者信息
Hashimoto H, Aino H, Ogawa N, Nagata S, Baba A
机构信息
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Biochem Biophys Res Commun. 1994 Apr 29;200(2):1042-8. doi: 10.1006/bbrc.1994.1555.
Parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor expressed in rat cultured cortical type I astrocytes was identified and characterized. Northern blot analysis demonstrated that the PTH/PTHrP receptor mRNA is expressed in astrocytes. A human PTH fragment, PTH [1-34], stimulated adenylate cyclase with the EC50 value of 3 nM, but not phospholipase C. PTH [1-34] changed the morphology of protoplasmic type astrocytes into process-bearing ones. Thus, type I astrocytes possess PTH/PTHrP receptor that is coupled to an adenylate cyclase-activating system but not to phospholipase C.
在大鼠培养的皮质I型星形胶质细胞中表达的甲状旁腺激素（PTH）/甲状旁腺激素相关肽（PTHrP）受体被鉴定和表征。Northern印迹分析表明，PTH/PTHrP受体mRNA在星形胶质细胞中表达。人PTH片段PTH [1-34]刺激腺苷酸环化酶，其EC50值为3 nM，但不刺激磷脂酶C。PTH [1-34]将原浆型星形胶质细胞的形态转变为有突起的细胞。因此，I型星形胶质细胞具有与腺苷酸环化酶激活系统偶联但不与磷脂酶C偶联的PTH/PTHrP受体。
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