Leone A M, Francis P L, Rhodes P, Moncada S
Wellcome Research Laboratories, Beckenham, Kent, UK.
Biochem Biophys Res Commun. 1994 Apr 29;200(2):951-7. doi: 10.1006/bbrc.1994.1542.
Quantification of nitric oxide in vivo is difficult because of its short half-life. Instead the oxidised products, nitrite and nitrate, are commonly used as indices of nitric oxide generation. We describe here a simple method for the measurement of nitrite and nitrate in plasma using high performance capillary electrophoresis. Plasma standard curves gave regression coefficients of 0.98 for nitrite and 0.99 for nitrate with an intra-assay coefficient of variance of 4.6% for 50 microM nitrite (n = 10) and 1.2% for 50 microM nitrate (n = 10). The assay allows measurement of nitrite and nitrate in a single analysis requiring minimal sample preparation, and with the sensitivity to detect both basal and physiological changes in plasma nitrite and nitrate.
由于一氧化氮半衰期短，因此体内一氧化氮的定量分析很困难。取而代之的是，氧化产物亚硝酸盐和硝酸盐通常被用作一氧化氮生成的指标。我们在此描述一种使用高效毛细管电泳测量血浆中亚硝酸盐和硝酸盐的简单方法。血浆标准曲线中亚硝酸盐的回归系数为0.98，硝酸盐的回归系数为0.99，50微摩尔亚硝酸盐的批内变异系数为4.6%（n = 10），50微摩尔硝酸盐的批内变异系数为1.2%（n = 10）。该检测方法能够在单次分析中测量亚硝酸盐和硝酸盐，所需样品制备最少，并且具有检测血浆中亚硝酸盐和硝酸盐基础水平及生理变化的灵敏度。
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