Cote G J
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Biochem Biophys Res Commun. 1994 Apr 29;200(2):993-8. doi: 10.1006/bbrc.1994.1548.
RNA transcripts derived from the calcitonin (CT)/calcitonin gene-related peptide (CGRP) gene are differentially processed in a tissue-specific fashion to produce two unique mRNAs. This RNA processing decision is deregulated in malignant thyroid C-cells. To examine this mechanism of RNA processing, CT/CGRP minigene constructs were transfected into the human medullary thyroid carcinoma TT cell line. RNA derived from the normal CT/CGRP construct paralleled the endogenous pathway to produce both CT and CGRP mRNAs. Mutation analysis and RNA/protein crosslinking were performed in order to clarify trans-acting factor interactions. The data suggest that CGRP production in TT cells results from the coexpression of facilitative and inhibitory factors.
源自降钙素（CT）/降钙素基因相关肽（CGRP）基因的RNA转录本以组织特异性方式进行差异加工，以产生两种独特的mRNA。这种RNA加工决定在恶性甲状腺C细胞中失调。为了研究这种RNA加工机制，将CT/CGRP微型基因构建体转染到人甲状腺髓样癌TT细胞系中。源自正常CT/CGRP构建体的RNA与内源性途径平行，以产生CT和CGRP mRNA。进行了突变分析和RNA/蛋白质交联，以阐明反式作用因子的相互作用。数据表明，TT细胞中CGRP的产生是促进因子和抑制因子共表达的结果。
