Potten C S, Booth C, Chadwick C A, Evans G S
Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Growth Factors. 1994;10(1):53-61. doi: 10.3109/08977199409019603.
The epithelium lining the small intestine is one of the most rapidly proliferating body tissues yet it rarely develops cancers. The proliferation, migration and differentiation of the stem cell progeny appears to be under very strict control. After 8 Gy gamma irradiation the murine epithelium contains surviving stem cells from which the epithelium rapidly and effectively regenerates, presumably in response to stimulatory signals, and then returns to steady state conditions after overshoots in proliferation. Here we describe the isolation and preliminary characterisation in vitro of a potent stimulatory extract obtained by diffusion from intact murine small intestine, post-irradiation. In addition to in vivo responses the extract stimulates intestinal epithelial lines very effectively, most notably the rat IEC 18 line where it can replace the serum requirement. The extent of the induced increase in proliferation could not be reproduced by any other single growth factor tested. Preliminary evidence suggests the extract contains either a potent stimulatory cocktail of factors or a novel intestinal growth factor(s).
小肠内衬上皮是人体中增殖最迅速的组织之一，但却很少发生癌变。干细胞后代的增殖、迁移和分化似乎受到非常严格的控制。8 Gy γ射线照射后，小鼠上皮中含有存活的干细胞，上皮细胞大概是对刺激信号作出反应，从而迅速有效地从这些干细胞再生，然后在增殖过度后恢复到稳态。在此，我们描述了一种通过从辐照后的完整小鼠小肠扩散获得的强效刺激提取物的体外分离和初步特性鉴定。除了体内反应外，该提取物还能非常有效地刺激肠上皮细胞系，最显著的是大鼠IEC 18细胞系，它可以替代血清需求。所测试的任何其他单一生长因子都无法重现诱导增殖增加的程度。初步证据表明，该提取物要么含有强效刺激因子混合物，要么含有一种新型肠道生长因子。
