Sotgia C, Fascio U, Ortolani G, De Bernardi F
Department of Biology, University of Milano, Italy.
Int J Dev Biol. 1993 Dec;37(4):547-53.
Swimming larvae of Phallusia mamillata are known to have "button cells" of endodermal origin between the ventral surface of the pharynx and the epidermis, that are stainable by various techniques. By immunofluorescence with anti-tubulin antibody and confocal laser microscopy, we obtained a bright reaction at one pole of the cells, suggesting the presence of a cap of tubulin and of microtubules overlaying the nucleus. During metamorphosis the microtubule-rich pseudopods at their base reach the epidermis, especially in the area near the adhesive papillae. Then they emigrate through the epidermis and become roundish again.
已知乳头海鞘的游动幼虫在咽的腹面和表皮之间有内胚层起源的“纽扣细胞”，这些细胞可用各种技术染色。通过用抗微管蛋白抗体进行免疫荧光和共聚焦激光显微镜观察，我们在细胞的一极获得了明亮的反应，表明存在覆盖细胞核的微管蛋白帽和微管。在变态过程中，它们基部富含微管的伪足到达表皮，尤其是在粘附乳头附近的区域。然后它们穿过表皮迁移并再次变圆。
