Zekorn T, Entenmann H, Horcher A, Siebers U, Klöck G, Zimmermann U, Federlin K, Bretzel R G
Medizinische Klinik III und Poliklinik, Justus-Liebig-Universität, Giessen, Germany.
Acta Diabetol. 1993;30(4):238-42. doi: 10.1007/BF00569935.
We have recently demonstrated long-lasting normoglycaemia after transplantation of barium alginate microencapsulated rat and porcine islets. Nevertheless the transplantation results obtained with different microencapsulation techniques have been controversial. Little is known about possible immune interactions between host and encapsulated islet. This study demonstrates in vitro stimulation of lymphoid cells by encapsulated islets that is similar to that of unencapsulated islets. This stimulation was reduced by a 4-day culture with unencapsulated islets only. After macroencapsulation of islets in hollow fibres a stimulatory effect was also observed, but this was less pronounced than after microencapsulation. Empty microcapsules as well as macrocapsules induced lymphoid proliferation as a result of mitogenic impurities in the encapsulation materials themselves. In the same donor-recipient combination in which we have shown successful transplantation, we found activation of the sensibilization arm of the immune system. This suggests that microencapsulation results in protection of the transplanted islets from the action of the effector arm. This lymphoid activation could be triggered by the mitogeniticity of the encapsulation material itself. In the case of alginates these mitogenic factors could not be abolished by culture (i.e. dialysis).
我们最近证明，移植钡藻酸盐微囊化大鼠和猪胰岛后可实现长期正常血糖。然而，不同微囊化技术获得的移植结果一直存在争议。关于宿主与包封胰岛之间可能存在的免疫相互作用，人们了解甚少。本研究表明，包封胰岛对淋巴细胞的体外刺激与未包封胰岛相似。仅与未包封胰岛进行4天培养后，这种刺激作用减弱。将胰岛在中空纤维中进行大囊封装后，也观察到了刺激作用，但不如微囊封装后明显。空微囊和大囊由于封装材料本身的促有丝分裂杂质而诱导淋巴细胞增殖。在我们已证明移植成功的同一供体-受体组合中，我们发现免疫系统的致敏分支被激活。这表明微囊封装可保护移植的胰岛免受效应分支的作用。这种淋巴细胞激活可能由封装材料本身的促有丝分裂活性引发。就藻酸盐而言，这些促有丝分裂因子不能通过培养（即透析）消除。
