Lessa E P, Applebaum G
Museum of Vertebrate Zoology, University of California, Berkeley 94720.
Mol Ecol. 1993 Apr;2(2):119-29. doi: 10.1111/j.1365-294x.1993.tb00006.x.
This article reviews four 'DNA screening techniques', namely heteroduplex analysis, single-strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.
本文综述了四种“DNA筛选技术”，即异源双链分析、单链构象多态性（SSCP）、变性梯度凝胶电泳（DGGE）和温度梯度凝胶电泳（TGGE），作为研究自然种群中等位基因变异的工具。讨论并比较了每种技术的分辨能力、优点和局限性。我们还提供了一些技术选择标准，并以主要来自我们自己实验室经验的实例来说明一些实际问题。
