来自尾草履虫纤毛的交配反应性膜囊泡。
Mating-reactive membrane vesicles from cilia of Paramecium caudatum.
作者信息
Kitamura A, Hiwatashi K
出版信息
J Cell Biol. 1976 Jun;69(3):736-40. doi: 10.1083/jcb.69.3.736.
Abstract
Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types.
摘要
通过用含有2 M尿素和0.1 mM Na2-EDTA的溶液处理，从尾草履虫的纤毛中获得了具有高交配反应性的膜泡。通过大约10微克/毫升的膜泡蛋白在互补交配型细胞中诱导了所有的接合过程。电子显微镜观察表明，膜泡的直径为100-150纳米。在SDS聚丙烯酰胺凝胶上的电泳分析显示，来自两种互补交配型的颗粒的多肽模式没有显著差异。
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