尾草履虫的纤毛膜与交配物质。
Ciliary membranes and mating substances in Paramecium caudatum.
作者信息
Watanabe T
出版信息
J Protozool. 1977 Aug;24(3):426-9. doi: 10.1111/j.1550-7408.1977.tb04767.x.
Cilia detached from mating reactive cells of Paramecium caudatum were fractionated for the purpose of identifying the structural component bearing mating substances. Purified axoneme fractions had no mating reactivity. The membrane fraction obtained by dialyzing against a solution of Tris-EDTA (0.1 mm EDTA, 1 mM Tris-HCI, pH 7.6) and 0.6 m KCI, and then by centrifuging over 40% (w/v) sucrose was strongly reactive. No mating reactivity was detected in the soluble fractions containing axonemal and matrix proteins. The results indicate that the mating substances in active form are localized only on the ciliary membranes.
为了鉴定携带交配物质的结构成分，对从尾草履虫交配反应性细胞上分离下来的纤毛进行了分级分离。纯化的轴丝级分没有交配反应性。通过用Tris-EDTA溶液（0.1 mM EDTA，1 mM Tris-HCl，pH 7.6）和0.6 M KCl透析，然后在40%（w/v）蔗糖上离心获得的膜级分具有强烈的反应性。在含有轴丝和基质蛋白的可溶性级分中未检测到交配反应性。结果表明，活性形式的交配物质仅定位在纤毛膜上。
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