苯乙酸诱导人横纹肌肉瘤细胞系发生肌源性分化。

Induction of myogenic differentiation in a human rhabdomyosarcoma cell line by phenylacetate.

作者信息

Cinatl J, Cinatl J, Herneiz P, Rabenau H, Hovak M, Benda R, Gümbel H O, Kornhuber B, Doerr H W

机构信息

Centre of Hygiene, Institute of Medical Virology, J.W. Goethe University, Frankfurt/Main, Germany.

出版信息

Cancer Lett. 1994 Apr 1;78(1-3):41-8. doi: 10.1016/0304-3835(94)90029-9.

DOI:10.1016/0304-3835(94)90029-9

PMID:8180967

Abstract

Sodium phenylacetate (NaPA) at concentrations ranging from 2 to 10 mM promoted myogenic differentiation of the human alveolar rhabdomyosarcoma cell line KFR. These concentrations inhibited DNA synthesis of the cells in a dose-dependent manner without significant effect on cell viability. The morphological differentiation of small mononuclear elements to terminal, elongated multinuclear structures resembling myotubes was accompanied by the expression of skeletal muscle myosin. The proportion of differentiated myosin-positive cells which was around 0.8-1.7% in control cultures 12 days after seeding was increased by NaPA treatment up to 47%. In the cytoplasm of differentiated cells, features of sarcomerogenesis were observed. These results suggest that NaPA is an effective inducer of rhabdomyosarcoma cell differentiation at concentrations that have been achieved in humans with no significant adverse effects.

摘要

浓度在2至10 mM范围内的苯乙酸钠（NaPA）可促进人肺泡横纹肌肉瘤细胞系KFR的成肌分化。这些浓度以剂量依赖性方式抑制细胞的DNA合成，而对细胞活力无显著影响。小单核细胞向类似肌管的终末伸长多核结构的形态分化伴随着骨骼肌肌球蛋白的表达。接种后12天，对照培养物中分化的肌球蛋白阳性细胞比例约为0.8 - 1.7%，经NaPA处理后该比例增加至47%。在分化细胞的细胞质中，观察到肌节形成的特征。这些结果表明，NaPA在已在人体中达到的浓度下是横纹肌肉瘤细胞分化的有效诱导剂，且无显著不良反应。