Cinatl J, Cinatl J, Mainke M, Weissflog A, Rabenau H, Kornhuber B, Doerr H W
Department of Medical Virology, J.W. Goethe-University, Frankfurt, Germany.
Cancer Lett. 1993 Jun 15;70(1-2):15-24. doi: 10.1016/0304-3835(93)90069-l.
Sodium phenylacetate (NaPA) at concentrations ranging from 2 to 6 mM stimulated morphological differentiation of two human neuroblastoma cell lines IMR-32 and UKF-NB-3. These concentrations inhibited growth and DNA synthesis of the cells in a dose dependent manner without significant effect on cell viability. The differentiated cells showed pseudoganglia formation and extension of cellular processes. The morphological differentiation in both cell lines was accompanied by decreased expression of N-myc oncoprotein. These results suggest that NaPA at concentrations, which have been achieved in humans with no significant adverse effects, promotes differentiation of cultured human neuroblastoma cells in association with the reduced expression of the malignant phenotype.
浓度在2至6毫摩尔范围内的苯乙酸钠（NaPA）刺激了两种人类神经母细胞瘤细胞系IMR - 32和UKF - NB - 3的形态分化。这些浓度以剂量依赖的方式抑制细胞的生长和DNA合成，而对细胞活力没有显著影响。分化后的细胞表现出假神经节形成和细胞突起的延伸。两种细胞系中的形态分化都伴随着N - myc癌蛋白表达的降低。这些结果表明，在人体内已达到的且无明显不良反应的浓度下，苯乙酸钠与恶性表型表达降低相关联，促进了培养的人类神经母细胞瘤细胞的分化。
