Yazaki K, Mimura T, Kawaguchi M
Department of Pharmacology, Tokyo Dental College, Japan.
Bull Tokyo Dent Coll. 1993 Aug;34(3):141-5.
We examined four kinds of drugs for dental use for their ability to enhance DNA synthesis and the proliferation activity of cells by means of measuring 3H-thymidine uptake into cells. Three phenolic compounds; guaiacol, eugenol and phenol, and trioxane, the trimer of the paraformaldehyde complex, were examined. The effects of variations in concentration of lower doses of the dental drugs on subsequent uptake of 3H-thymidine were examined in osteogenic cells and fibroblastic cells. Osteogenic cells from human spongy bone, fibroblastic cells from human periodontal ligament, and mouse osteogenic MC3T3-E1 cells were used. 3H-thymidine uptake of osteogenic cells or fibroblastic cells was depressed in a concentration dependent manner by doses over 10(-6) M of the phenolic compounds. Over 10(-6) M, eugenol and guaiacol depressed the uptake of osteogenic cells or fibroblastic cells significantly and depressed it almost completely at doses over 10(-4) M. Phenol depressed the cells significantly over 10(-4) M. Trioxane depressed at all doses from 10(-4) M to 10(-16) M. The phenolic compounds enhanced the uptake of 3H-thymidine in a concentration dependent manner at doses below 10(-8) M of the phenolic compounds. The cells were enhanced in 3H-thymidine uptake maximally at 10(-12) M or 10(-14) M concentrations of the phenolic compounds.
我们通过测量细胞对³H - 胸腺嘧啶核苷的摄取，研究了四种牙科用药增强DNA合成及细胞增殖活性的能力。研究对象包括三种酚类化合物：愈创木酚、丁香酚和苯酚，以及多聚甲醛复合物的三聚体三聚甲醛。在成骨细胞和成纤维细胞中，研究了低剂量牙科用药浓度变化对随后³H - 胸腺嘧啶核苷摄取的影响。使用了来自人松质骨的成骨细胞、来自人牙周韧带的成纤维细胞以及小鼠成骨MC3T3 - E1细胞。当酚类化合物剂量超过10⁻⁶ M时，成骨细胞或成纤维细胞对³H - 胸腺嘧啶核苷的摄取呈浓度依赖性降低。超过10⁻⁶ M时，丁香酚和愈创木酚显著降低成骨细胞或成纤维细胞的摄取，在剂量超过10⁻⁴ M时几乎完全抑制。苯酚在超过10⁻⁴ M时显著抑制细胞。三聚甲醛在10⁻⁴ M至10⁻¹⁶ M的所有剂量下均有抑制作用。在酚类化合物剂量低于10⁻⁸ M时，酚类化合物以浓度依赖性方式增强³H - 胸腺嘧啶核苷的摄取。在酚类化合物浓度为10⁻¹² M或10⁻¹⁴ M时，细胞对³H - 胸腺嘧啶核苷的摄取增强最大。
