Kang C, Chitnis P R, Smith S, Krogmann D W
Biochemistry Department, Purdue University, W. Lafayette, IN 47907.
FEBS Lett. 1994 May 9;344(1):5-9. doi: 10.1016/0014-5793(94)00341-6.
The gene for the low potential cytochrome c (petK) was isolated from a genomic library of Synechocystis PCC 6803. The nucleotide sequence of this gene contains two regions with sequence similarity to two regions in the gene for the high potential cytochrome c6 of the organism. The sixth iron ligand can be identified with a conserved histidine. Experiments demonstrate the reduction of the low potential cytochrome by reduced ferredoxin II. The heme of the cytochrome is flanked by lysines which may be involved in orienting the ferredoxin near the site of electron donation.
从集胞藻PCC 6803的基因组文库中分离出低电位细胞色素c（petK）基因。该基因的核苷酸序列包含两个区域，其与该生物体高电位细胞色素c6基因中的两个区域具有序列相似性。第六个铁配体可通过一个保守的组氨酸来鉴定。实验证明还原型铁氧化还原蛋白II可还原低电位细胞色素。细胞色素的血红素两侧是赖氨酸，它们可能参与在电子供体位点附近定位铁氧化还原蛋白。
