在两种不同的胰高血糖素原加工细胞系中对激素原转化酶进行免疫检测。

Immunological detection of prohormone convertases in two different proglucagon processing cell lines.

作者信息

Blache P, Le-Nguyen D, Boegner-Lemoine C, Cohen-Solal A, Bataille D, Kervran A

机构信息

Institut National de la Santé et de la Recherche Médicale, Unité 376, CCIPE, Montpellier, France.

出版信息

FEBS Lett. 1994 May 9;344(1):65-8. doi: 10.1016/0014-5793(94)00371-8.

DOI:10.1016/0014-5793(94)00371-8

PMID:8181567

Abstract

The distribution of the prohormone convertases, PC1/3, PC2 and PC5/6, was determined by immunoblotting in two cell lines. In alpha TC1-6 cells, the proglucagon processing occurred according to the pancreatic A-cell type. In STC-1 cells, proglucagon was processed in a manner reminiscent of the intestinal L-cell type. PC1/3 was undetectable in both proglucagon processing cell lines whereas PC2 displayed a strong immunostaining in the alpha TC1-6 cells and was barely detectable in the STC-1 cells. PC5/6 was detected as a 70 kDa protein in both cell lines. These results suggest a possible role of PC2 in the processing of proglucagon into glucagon in the A-cells, whereas in L-cells it would require still undetermined endoproteases.

摘要

通过免疫印迹法在两种细胞系中测定了激素原转化酶PC1/3、PC2和PC5/6的分布。在αTC1-6细胞中，胰高血糖素原的加工过程符合胰腺A细胞类型。在STC-1细胞中，胰高血糖素原的加工方式让人联想到肠道L细胞类型。在这两种胰高血糖素原加工细胞系中均未检测到PC1/3，而PC2在αTC1-6细胞中显示出强烈的免疫染色，在STC-1细胞中几乎检测不到。在两种细胞系中均检测到PC5/6为一种70 kDa的蛋白质。这些结果表明，PC2可能在A细胞中将胰高血糖素原加工成胰高血糖素的过程中发挥作用，而在L细胞中，这一过程则需要尚未确定的内切蛋白酶。