Ward L J, Brown J C, Davey G P
New Zealand Dairy Research Institute, Palmerston North.
FEMS Microbiol Lett. 1994 Mar 15;117(1):29-33. doi: 10.1111/j.1574-6968.1994.tb06738.x.
This paper reports on the application of the ligase chain reaction (LCR) to the specific detection of variants of the nisin structural gene (nisinA and nisinZ) in nisin producing strains of Lactococcus lactis ssp lactis. The LCR assay was used to screen nisin producing strains to determine which form of the nisin structural gene they contained. This method of differentiating the nisin structural gene variants provides a useful alternative to the only other available genetic differentiation, that of sequencing the gene.
本文报道了连接酶链式反应（LCR）在乳酸乳球菌乳酸亚种产乳链菌肽菌株中乳链菌肽结构基因（乳链菌肽A和乳链菌肽Z）变体特异性检测中的应用。LCR分析用于筛选产乳链菌肽菌株，以确定它们所含乳链菌肽结构基因的形式。这种区分乳链菌肽结构基因变体的方法为唯一可用的其他基因鉴别方法（即对该基因进行测序）提供了一种有用的替代方法。
