Albertson N H, Nyström T
Department of General and Marine Microbiology, University of Göteborg, Sweden.
FEMS Microbiol Lett. 1994 Apr 1;117(2):181-7. doi: 10.1111/j.1574-6968.1994.tb06762.x.
The decay rate of the potential to synthesize proteins after complete inhibition of transcription by rifampicin was analyzed to determine the functional mRNA stability of exponentially growing and glucose-starved Escherichia coli B and K12 cells. We found the following: (i) The half-life of the mRNA pool increased 2.2-fold during a period of 2 h of starvation (from 1.8 min in exponentially growing cells to 4.0 min for cells starved for 2 h); (ii) the effect on transcript stability appeared to be global since transcripts of genes that were induced, repressed or unaltered in their expression during starvation exhibited more or less the same increased stability; (iii) the rate of peptide chain elongation, as measured by the synthesis time for beta-galactosidase, decreased 1.9-fold during the starvation period studied and may, at least in part, account for the global stabilization of transcripts in starved cells.
通过利福平完全抑制转录后，分析了指数生长和葡萄糖饥饿的大肠杆菌B和K12细胞合成蛋白质潜能的衰减率，以确定功能性mRNA的稳定性。我们发现：(i) 在饥饿2小时期间，mRNA池的半衰期增加了2.2倍（从指数生长细胞中的1.8分钟增加到饥饿2小时细胞中的4.0分钟）；(ii) 对转录本稳定性的影响似乎是全局性的，因为在饥饿期间表达被诱导、抑制或未改变的基因的转录本表现出或多或少相同程度的稳定性增加；(iii) 通过β-半乳糖苷酶的合成时间测量的肽链延伸速率，在研究的饥饿期间下降了1.9倍，并且可能至少部分地解释了饥饿细胞中转录本的全局稳定性。
