Fukui K, Kamisugi Y, Sakai F
Department of Breeding, Hokuriku National Agricultural Experiment Station, Joetsu, Japan.
Genome. 1994 Feb;37(1):105-11. doi: 10.1139/g94-013.
5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simultaneous direct labeling in PCR. Four 5S rDNA loci were detected in a haploid genome by the 5S rDNA coding region, whereas in addition, the four or six 5S rDNA related sites, depending on the variety used, were revealed by the probe covering the flanking region. The four 5S rDNA loci revealed and mapped on the barley chromosomes: 2 (2I), 3 (3I), 1 (7I), and 4 (4I) were designated 5SRrn-I1, 5SRrn-I2, 5SRrn-I3 and 5SRrn-I4, respectively, in descending order of copy number of 5S rRNA genes.
利用五条相互易位系，通过原位杂交将5S核糖体DNA（rDNA）基因座定位在大麦染色体上。使用了两种DNA探针，一种覆盖5S rDNA编码区，另一种覆盖5S rDNA编码区及其侧翼非编码区。它们是通过从间期核中直接克隆并在PCR中同时进行直接标记制备的。通过5S rDNA编码区在单倍体基因组中检测到四个5S rDNA基因座，而此外，根据所用品种的不同，覆盖侧翼区域的探针还揭示了四个或六个与5S rDNA相关的位点。在大麦染色体上揭示并定位的四个5S rDNA基因座：2（2I）、3（3I）、1（7I）和4（4I），按照5S rRNA基因拷贝数从高到低的顺序，分别命名为5SRrn-I1、5SRrn-I2、5SRrn-I3和5SRrn-I4。
